Journal: Journal of Cellular and Molecular Medicine

Article Title: Generation of ‘designer erythroblasts’ lacking one or more blood group systems from CRISPR/Cas9 gene‐edited human‐induced pluripotent stem cells

doi: 10.1111/jcmm.16872

Figure Lengend Snippet: CRISPR/Cas9 gene‐edited erythroblasts surface antigen presentation. Complete loss of RhAG, RhD/CE, GPB protein expression was confirmed for RhAG, RhD/CE (A) and GPB (B) erythroblasts. Anti‐U was used to confirm GPB deletion. Kell glycoprotein expression was evaluated as a proxy for XK knockout erythroblasts. Kell expression (C) was considerably diminished consistently with the lack of Kx expression. KO, knockout

Article Snippet: Briefly, cloning was performed by ligating annealed‐guides into BbsI digested GFP‐Cas9 expression vector pX458 (one guide/vector) using a quick ligation kit (New England Biolabs, Ipswich, MA, USA) and transformed into competent E. coli cells (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: CRISPR, Expressing, Knock-Out

Journal: Molecular Cell

Article Title: Circ-ZNF609 Is a Circular RNA that Can Be Translated and Functions in Myogenesis

doi: 10.1016/j.molcel.2017.02.017

Figure Lengend Snippet:

Article Snippet: The oligonucleotides (Flag_zfp_guide2_FW and Flag_zfp_guide2_RV) encoding the Cas9 guide RNA were annealed and ligated in a BbsI-digested px330 vector (addgene #42230, ( )).

Techniques: Recombinant, Knock-Out, Electron Microscopy, DNA Extraction, Labeling, Clone Assay, Immunoprecipitation, Luciferase, RNA Sequencing Assay, Plasmid Preparation, Software

Journal: iScience

Article Title: Dissection of the MEF2D-IRF8 transcriptional circuit dependency in acute myeloid leukemia

doi: 10.1016/j.isci.2022.105139

Figure Lengend Snippet:

Article Snippet: For murine Cas9 sgRNAs, the oligos were ligated into the BbsI digested pSL21-mCherry (Addgene:164,410) vector.

Techniques: Recombinant, Reverse Transcription, Magnetic Beads, Protease Inhibitor, Cell Viability Assay, Cloning, Purification, Isolation, Sequencing, Software